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. Author manuscript; available in PMC: 2016 May 9.
Published in final edited form as: J Immunol. 2007 Nov 15;179(10):6770–6782. doi: 10.4049/jimmunol.179.10.6770

FIGURE 9. Biosynthesis of TGF-β1 by macrophages after phagocytosis of SM4s-painted late apoptotic C26 cells.

FIGURE 9.

A, Macrophages were grown in 6-well plates, incubated with control and SM4s-painted apoptotic C26 cells for 4 h and 12 h, and subjected to immunostaining for intracellular TGF-β1. Representative contour plots with percent of TGF-β1-positive macrophages 4 h after start of coincubation. B, Statistical analysis with time course of intracellular total TGF-β1 for 4 h and 12 h time points. C, Macrophages were grown in 6-well plates and cocultured with apoptotic C26 cells, SM4s-positive C26 cells, SM4s (10 µM), or vehicle at 37°C for 12 h. Supernatants were collected and subjected to ELISA. Data represent mean ± SEM of three experiments in duplicates. Statistical differences were evaluated by paired t test. **, p < 0.01.