Fig. 4.

Characterization of human apoA-I HDL was isolated by density gradient ultracentrifugation, dialyzed, and delipidated apolipoproteins were separated by size exclusion chromatography as described in Materials and methods. (A) SDS–PAGE (12%) of purified human apoA-I (10 μg) and HDL (containing apoA-I and apoA-II; 10 μg protein) was performed under the conditions described in Materials and methods. The molecular mass of the marker proteins is indicated. (B) ApoA-I (5 μg) was separated by RP–HPLC (Vydac C 18 column) and analyzed by ESI–MS.