Fig. 5.

Characterization of apoA-I-coated proticles The binding capacity of proticles was determined using Cy-3-labeled apoA-I. Proticles were incubated in the presence of indicated amounts of apoA-I (1 h, ambient temperature, 300 rpm) and centrifuged (20,000×g, 2 h, 4 °C). 100 μl aliquots were removed from the supernatant and fluorescence determined using a fluorescence plate reader (544/590 nm, Ex/Em). The percentage of apoA-I bound to proticles (filled squares) was calculated from the difference in fluorescence intensities measured prior to and after the coincubation of proticles and Cy-3-labeled apoA-I. Effects of apoA-I conjugation on particle size were determined by dynamic light scattering prior and after the incubation of proticles in the absence (filled triangles) or presence (open triangles) of the indicated mass ratios of apoA-I/ON. Results are mean±S.D. from three different experiments performed in triplicates.