Fig. 6.

ApoA-I coating of proticles does not affect BCEC viability, results in increased uptake rates and alters subcellular distribution of proticles in BCEC monolayers. (A) BCEC were incubated in the presence of the indicated concentration of proticles or apoA-I-coated proticles and cell viability was determined as described in the legend to Fig. 1C. Results shown are mean±S.D. (n = 4). (B) BCEC were incubated in the presence of ¹²⁵I-labeled proticles and ¹²⁵I-labeled apoA-I-coated proticles (2.5 μg/ml) for the indicated times. Cells were washed, lysed in NaOH (0.3 M) at 4 °C for 8 h and the radioactivity was counted. An aliquot of the lysate was used to determine the cellular protein content. Results shown are mean±S.D. (n = 3). **p<0.05;***p<0.005. (C) BCEC were cultured on chamber slides and incubated with Cy-5-labeled uncoated or apoA-I-coated proticles (shown in blue) for 4 h. Following the incubations (4 h) cells were fixed and VE-cadherin staining (red) was performed as described in Materials and methods. Detection was performed with a Cy-3-labeled goat anti-mouse antibody.