Fig. 7.

ApoA-I coating of proticles results in enhanced transcytosis rates and increased uptake by astrocytes in a coculture model of the BBB. (A) BCEC were cultured on polycarbonate filter inserts and CCF–STT astrocytes were cultured on the bottom of the basolateral compartment. After induction of tight junctions, uncoated or apoA-I-coated ¹²⁵I-labeled proticles were added to the apical medium was removed for counting of the radioactivity and replaced with fresh medium. Results are mean±S.D. (n = 3). (B) After 4 h the polycarbonate filter inserts containing BCEC were washed, cut out, transferred to a new insert and lysed in NaOH (0.3 M) to determine the cell-associated radioactivity. An aliquot was used to determine the cellular protein content. Astrocytes were washed, lysed in NaOH and processed as described for BCEC. Results are mean±S.D. (n = 3). **p<0.05.