Fig. 8.

Transcytosis of apoA-I-coated ¹²⁵I-proticles is reduced in the presence of an SR-BI neutralizing antibody BCEC were cultured on collagen-coated 12-well Transwell clusters. Where indicated a polyclonal blocking antiserum (1 μl) against SR-BI was added to the apical compartment 60 min prior to the addition of 2.5 μg of apoA-I-coated ¹²⁵I-proticles/ml to the apical compartment. Uncoated ¹²⁵I-proticles were used as controls (2.5 μg/ml). Cells were incubated at 37 °C and at indicated time points, 200 μl aliquots were removed from the basolateral compartment, replaced with fresh medium, and the radioactivity was determined by γ-counting. Data shown represent mean±S.D. from one experiment performed in triplicates.