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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: ACS Comb Sci. 2016 Mar 3;18(3):139–143. doi: 10.1021/acscombsci.5b00195

Figure 5.

Figure 5

Final eluted peptide products for representative reaction series X1Y1-X4Y1. A dual-clicked peptide product can be obtained without an intermediate purification step between CuAAC reactions. RP-HPLC traces monitored at 214 nm, on a 30 min gradient from 100% H2O with 0.05% TFA (Buffer A) to 90% ACN, 10% H2O with 0.05% TFA (Buffer B).