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Selective labeling of the CX10R7-tagged membrane protein OmpX on E. coli surface with biotin-PEG4-CBT. (a) Labeling scheme. (b) Micrographs of E. coli expressing either OmpX-A7 or OmpX-CX10R7. The cells were treated with 500 μM biotin- PEG4-CBT for 1 h before PBS wash and subsequent staining with streptavidin-Alexa Fluor 568 for 45 min. The last row shows the 3× digitally zoomed-in images. Scale bar = 2 μm.
