Table 1. Proportion of CD4⁺Foxp3⁺ Tregs of CD4⁺ cells in spleen and draining lymph nodes before and after immunization.

Tissue	Mice	Day
		0	3	5	28	49
Spleen	LNT-Ctrl	26.1 ± 0.6	22.6 ± 0.6	25.9 ± 0.2	28.0 ± 1.2	21.5 ± 1.6
Spleen	LNT-CII	25.5 ± 1.8	26.1 ± 0.2**	24.2 ± 0.3*	24.1 ± 1.0*	21.1 ± 1.2
Lymph nodes	LNT-Ctrl	11.8 ± 0.9	12.0 ± 0.8	12.6 ± 0.7	ND	ND
Lymph nodes	LNT-CII	12.37 ± 0.4	14.9 ± 0.6*	11.7 ± 0.9	ND	ND

*p<0.05 and

**p<0.01 LNT-Ctrl vs LNT-CII. Data presented as mean ± SEM. At day 0, 3, 5 and 28 n = 3, at day 49 n = 5–6 per group. ND; not done.