Table 2. List of the primers used in this study.
| Technique | Target microbe | Primer pair | Sequence (5’ to 3’ direction) | References |
|---|---|---|---|---|
| Quantitative real-time PCR | Methanogens | qmcra-F | TTCGGTGGATCDCARAGRG C | [34] |
| qmcra-R | GBARGTCGWAWCCGTAGAATCC | |||
| Bacteria | 1114F | CGGCAACGAGCGCAACCC | [35] | |
| 1275R | CCATTGTAGCACGTGTGTAGCC | |||
| Diversity | Protozoa | PSSU-316F | GCTTTCGWTGGTAGTGTATT | [36] |
| PSSU-539R | CTTGCCCTCYAATCGTWCT | |||
| Methanogens | 340F | CCCTAYGGGGYGCASCAG | [37] | |
| 1000R | GAGARGWRGTGCATGGCC | |||
| Bacteria | 27F | AGAGTTTGATCCTGG | [38] | |
| 519R | TTACCGCGGCTGCT |