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. Author manuscript; available in PMC: 2016 May 9.
Published in final edited form as: Science. 2015 Jun 19;348(6241):1372–1376. doi: 10.1126/science.aab1223

Fig. 3. PHA-4 is required for Pol II occupancy at foregut genes.

Fig. 3

(A) Pol II occupancy at mig-38 (poised, ubiquitous) versus ceh-22 and T06D8.3 (poised, foregut) in smg-1 control versus pha-4(ts) embryos, normalized to eft-3 (set at 1) and srw-99 (set at 0). Error bars indicate n = 3 replicates, mean ± SEM. (B) mRNA abundance (quantitative RT-PCR) for mig-38, ceh-22, and T06D8.3 for wild-type or pha-4(ts) embryos early (E) or mid (M). n = 3 replicates, mean ± SEM. (C) Gene expression profiles from (20). Early stage (dark gray arrowhead) is at the fifth and sixth AB-div. Mid-stage (light gray arrowhead) is equivalent to ventral enclosure.