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. 2016 May 9;10(5):e0004704. doi: 10.1371/journal.pntd.0004704

Table 3. Number of patients found positive by DENV.

All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 99 patients admitted at Mahosot Hospital.

A Serum on RDT Neat serum
Positive Negative Total
Positive 41 1 42
Negative 7 50 57
Total 48 51 99
Overall agreement (95%CI): 91.9 (84.7–96.4)%
Positive agreement (95%CI): 85.4 (72.2–93.9)%
Negative agreement (95%CI): 98.0 (89.6–99.9)%
B Whole blood on RDT Neat serum
Positive Negative Total
Positive 34 3 37
Negative 14 48 62
Total 48 51 99
Overall agreement (95%CI): 82.8 (73.9–89.7)%
Positive agreement (95%CI): 70.8 (55.9–83.0)%
Negative agreement (95%CI): 94.1 (83.8–98.8)%
C Serum on filter paper Neat serum
Positive Negative Total
Positive 34 0 34
Negative 14 51 65
Total 48 51 99
Overall agreement (95%CI): 85.9 (77.4–92.0)%
Positive agreement (95%CI): 70.8 (55.9–83.0)%
Negative agreement (95%CI): 100 (93.0–100)%
D Whole blood on filter paper Neat serum
Positive Negative Total
Positive 35 0 35
Negative 13 51 64
Total 48 51 99
Overall agreement (95%CI): 86.9 (78.6–92.8)%
Positive agreement (95%CI): 72.9 (58.2–84.7)%
Negative agreement (95%CI): 100 (93.0–100)%

A: Serum on RDT compared to neat serum extraction. B: Whole blood on RDT compared to neat serum extraction. C: serum on filter paper compared to neat serum extraction. D: Whole blood on filter paper compared to neat serum extraction.