TABLE 1.
Effect of domain deletions on RE binding to ΔID
| Relative bindinga |
|||
|---|---|---|---|
| GST-RE14 | GST-RE24 | GST-RE34 | |
| ΔID | 1.00 | 1.00 | 1.00 |
| ΔID-ΔCARD | 0.91 | 0.07 | 0.11 |
| ΔID-ΔLATCH | 1.15 | 0.08 | 1.62 |
| ΔID-ΔCC | 0.05 | 0.39 | 0.42 |
| ΔID-ΔPDZ | 0.79 | 1.30 | 1.27 |
| ΔID-ΔL3 | 1.54 | 0.41 | 1.26 |
| ΔID-ΔSH3 | 2.88 | 1.17 | 1.54 |
| ΔID-ΔL4 | 0.70 | 1.30 | 0.87 |
| ΔID-ΔGUK | 0.23 | 0.75 | 1.15 |
a The relative binding in each column was calculated by determining the bound/input ratio for each CARD11 variant and normalizing to that observed with the ΔID after densitometric analysis of the blots in Fig. 6.