TABLE 2.
Effect of oncogenic mutations on RE binding to ΔID
| Relative bindinga | |||
|---|---|---|---|
| GST-RE14 | GST-RE24 | GST-RE34 | |
| ΔID | 1.00 | 1.00 | 1.00 | 
| ΔID-C49Y | 0.33 | 0.06 | 0.26 | 
| ΔID-F97Y | 0.46 | 0.05 | 0.63 | 
| ΔID-G123D | 0.12 | 0.05 | 0.16 | 
| ΔID-F130I | 0.38 | 0.20 | 0.15 | 
| ΔID-L232LI | 0.23 | 0.73 | 0.53 | 
a The relative binding in each column was calculated by determining the bound/input ratio for each CARD11 variant and normalizing to that observed with the ΔID after densitometric analysis of the blots in Fig. 7.