TABLE 3.

Effect of TIR-199 and other syrbactins on cancer cell death

BTZ was used as a control for comparison. MYCN2, neuroblastoma cell line SH-EP, which was previously transfected with a tetracycline on/off plasmid system to allow for controlled MYCN expression (33). SH-EP, S-type subclone of the cell line SK-N-SH. Data represent the mean values of three independent experiments, each performed in duplicate wells (n = 6). Values are either derived from this study (Fig. 4, G and H) or are historic values from the published literature. ND, not determined.

Cell line	IC50a
	TIR-199	SylAb,c	GlbAc	SylA-GlbAd	BTZc
	nm
SK-N-SH	100e	25,000	94	321	4.8
MM1.S	ND	8,500	4	28	2.4
MM1.RL	<50	39,300	5	27	3.0
U266	ND	ND	548	45	3.4
SKOV-3	ND	20,000	852	109	39.9

^a Inhibitory concentration at which cell viability is reduced by 50%.

^b Value from Ref. 11.

^c Value from Ref. 14.

^d Value from Ref. 13.

^e Instead of SK-N-SH, the MYCN2 cell line was used in experiments with TIR-199.