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. 2016 Feb 17;291(16):8363–8373. doi: 10.1074/jbc.M115.699546

FIGURE 1.

FIGURE 1.

DHCR7 is rapidly turned over compared with DHCR24. A, DHCR7 and DHCR24 catalyze the final steps of cholesterol synthesis via the Kandutsch-Russell and Bloch pathways, respectively. 7-Dehydrocholesterol can be converted to cholesterol by acting as a substrate for DHCR7 or to vitamin D through exposure to ultraviolet B (UVB) light. A simplified version of the modified Kandutsch-Russell pathway is shown as described in detail previously (2). B, CHO-DHCR24-V5 cells were transfected with empty vector (EV) or DHCR7-myc plasmid and treated with or without 10 μg/ml cycloheximide (CHX) for 0–8 h. Protein levels were analyzed using Western blotting with myc, V5, and GAPDH antibodies. Blots are representative of three independent experiments. There is a nonspecific band at 37 kDa in the myc immunoblot.