TABLE 1.
NMR chemical shifts of S. suis serotype 1 native CPS
Shown are chemical shifts (1H/13C) in 33 mmol/liter phosphate buffer, pD 8.0, in D2O in ppm referenced to internal DSS. 1H chemical shifts were obtained at 70 °C from the one-dimensional spectrum, at 65 °C from the COSY or TOCSY spectrum, or at 70 °C from the HSQC spectrum. 13C chemical shifts were obtained at 70 °C from the one-dimensional spectrum.
| Residue | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Me | CO | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | →3)-β-d-Gal-(1→ | 4.49 (7.8)a | 3.71 | 3.80 | 4.17 | 3.73 | 3.77 | ||||||||
| 105.30b | 72.87b | 84.81 | 71.06 | 77.65 | 63.68 | ||||||||||
| B | →4)-β-d-Glc-(1→ | 4.49 (7.8) | 3.33 | 3.63 | 3.64 | 3.58 | 3.97 | 3.80 | |||||||
| 105.29b | 75.54 | 77.19 | 81.28 | 77.51 | 63.00 | ||||||||||
| C | →6)-β-d-GalNAc-(1→ | 4.52 (8.4) | 3.93 | 3.74 | 3.93 | 3.79 | 4.00 | 3.61 | 2.06 | ||||||
| 104.77 | 55.24 | 73.43 | 70.28 | 76.42 | 66.06 | 24.97 | 177.30 | ||||||||
| D | →3,6)-β-d-Gal-(1→ | 4.62 (7.5) | 3.66 | 3.73 | 4.13 | 3.85 | 4.02 | 3.85 | |||||||
| 106.83 | 72.88b | 84.49 | 70.97 | 75.85 | 71.47 | ||||||||||
| E | →4)-β-d-GlcNAc-(1→ | 4.74 (7.5) | 3.78 | 3.78 | 3.55 | 3.54 | 3.82 | 3.64 | 2.05 | ||||||
| 105.14 | 57.66 | 75.11 | 83.54 | 76.89 | 63.03 | 25.08 | 177.49 | ||||||||
| F | α-d-Neu5Ac-(2→ | 2.68 | 1.68 | 3.68 | 3.77 | 3.71 | 3.56 | 3.88 | 3.87 | 3.65 | 2.03 | ||||
| 175.97 | 103.00 | 42.86 | 70.87 | 54.74 | 75.30 | 71.31 | 74.46 | 65.56 | 24.77 | 177.66 |
a Coupling constants (JH-1–H-2) in parentheses.
b Tentative assignments.