TABLE 2.
NMR chemical shifts of S. suis serotype 1/2 desialylated polysaccharide
Shown are chemical shifts (1H/13C) in D2O in ppm referenced to internal DSS. 1H chemical shifts were obtained at 56 °C from the one-dimensional, COSY, TOCSY, or HSQC spectrum. 13C chemical shifts were obtained at 56 °C from the one-dimensional or HSQC spectrum.
| Residue | 1 | 2 | 3 | 4 | 5 | 6 | Me | CO | ||
|---|---|---|---|---|---|---|---|---|---|---|
| A | β-d-GalNAc-(1→ | 4.53 (8.3)a | 3.92 | 3.75 | 3.94 | 3.72 | 3.78 | 2.06 | ||
| 104.47 | 55.33 | 73.43 | 70.40 | 78.01 | 63.64 | 24.96 | 177.39 | |||
| B | →3,4)-β-d-Gal-(1→ | 4.66 (7.3) | 3.65 | 3.77 | 4.35 | 3.68 | 3.78 | 3.76 | ||
| 105.67 | 73.2b | 85.13 | 77.8b | 76.9b | 63.32 | |||||
| C | →4)-β-d-GlcNAc-(1→ | 4.74 (7.8) | 3.76 | 3.73 | 3.61 | 3.52 | 3.85 | 3.66 | 2.03 | |
| 105.55 | 57.99 | 75.19 | 82.16 | 77.22 | 63.07 | 25.10 | 177.35 | |||
| D | →3,4)-β-l-Rha-(1→ | 4.86 | 4.39 | 3.93 | 3.87 | 3.50 | 1.35 | |||
| 103.1b | 68.40 | 77.99 | 78.6b | 73.84 | 19.94 | |||||
| E | →4)-β-d-Glc-(1→ | 4.87 | 3.32 | 3.66 | 3.61 | 3.49 | 3.93 | 3.79 | ||
| 104.7b | 76.3b | 78.4b | 80.0b | 77.15 | 63.81 | |||||
| F | α-d-Gal-(1→ | 5.25 (3.4) | 3.89 | 3.97 | 3.97 | 4.20 | 3.73 | |||
| 95.80 | 70.95 | 72.24c | 72.14c | 73.79 | 63.91 |
a Coupling constants (JH-1–H-2) in parentheses.
b Obtained from the HSQC spectrum.
c Tentative assignments.