FIGURE 11.
CatC in the BALF of rodents and macaques. A, initial rates of hydrolysis of the CatC substrate Gly-Phe-AMC (50 μm) by BALF from mice, rats, and macaques before and after incubation with the CatC diazomethyl ketone inhibitor Gly-Phe-CHN2 (25 μm). CatC activity was detected in the control BALF of rodents but not in that of macaques (n = 5) before LPS treatment. FU, fluorescence unit. B, immunoblotting of BALF from rodents and macaques showing the active CatC heavy chain in the BALF of rodents and that of LPS-treated macaques. Similar results were observed in three independent experiments.