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. 2016 Feb 16;291(16):8486–8499. doi: 10.1074/jbc.M115.707109

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Intracellular localization and secretion of mature CatC by activated cells. A, sideward (SS) versus forward (FS) scatter plot of undifferentiated (left panel) and differentiated PLB-985 cells (right). B, confocal microscopy of undifferentiated (left) and differentiated (right) PLB-985 cells immunostained with anti-CatC (Ab1) antibodies (green) and anti-golgin-84 antibodies (red) showing the initial localization of CatC in the Golgi of immature cells and its distribution throughout the cell after differentiation. C, CatC activity in cell-free supernatants of undifferentiated and differentiated PLB-985 cells after treatment with the calcium ionophore A23187. FU, fluorescence unit. D, immunoblot analysis of 20- or 80-fold concentrated supernatants of neutrophils (S1 to S7) after their activation with A23187 using the anti-CatC antibody Ab1. Similar results were found in three independent experiments.