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. 2016 Feb 16;291(16):8486–8499. doi: 10.1074/jbc.M115.707109

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Active PR3 in PLB-985. A, immunodetection of purified PR3, PR3 in PLB-985, HL-60, and neutrophil lysates using Bt-[PEG]₆₆-PYDA^P(O-C₆H₄-4-Cl)₂, a selective biotinylated probe of proteolytically active PR3. PMNs, polymorphonuclear neutrophils. B, RP-HPLC fractionation of cleavage products on a C18 cartridge after incubating the PR3 substrate Abz-VADnVADYQ-EDDnp with a PLB-985 cell lysate. Peak 1 is Abz-VADnV, and 2 is ADYQ-EDDnp. Similar results were observed in three independent experiments.