α-Syn-containing lipoprotein particles are well defined in size.
A, size-exclusion gel chromatography (Superdex 200 10/300GL) of α-Syn DOPS (black), α-Syn POPS (green), and α-Syn sphingomyelin (red) lipoprotein particles. Monomeric (gray) α-Syn elutes at ∼14.3 ml. B, dynamic light scattering of α-Syn-containing DOPS lipoprotein particles. The size-exclusion chromatography fraction at the maximum of the absorbance of 280 nm (9.5–10 ml) consists of particles with an average hydrodynamic radius of 25.8 ± 0.8 nm. C–E, negatively stained electron micrographs containing the maxima of the size-exclusion chromatography fractions at the absorbance of 280 nm of purified (C) α-Syn DOPS, (D) α-Syn POPS, and (E) α-Syn sphingomyelin lipoprotein particles. The radius varies from ∼19–28 nm, scale bar 100 nm.