Skip to main content
. 2016 Feb 4;291(16):8516–8527. doi: 10.1074/jbc.M115.707968

FIGURE 6.

FIGURE 6.

α-Syn DOPS lipoprotein particles comprise 8–10 copies of α-Syn as evidenced by cross-linking studies. SDS-PAGE, 4–12% NuPAGE Bis-Tris gel (Invitrogen), of cross-linked α-Syn DOPS lipoprotein particles. A, lane 1, molecular weight marker (MW, Precision Plus ProteinTM Dual Color Standard, BIORAD). Lane 2, (−) Non-cross-linked α-Syn DOPS lipoprotein particles (control). Lanes 3–8, α-Syn DOPS lipoprotein particles (final concentration 83 μm) were exposed to increasing concentrations (molar ratios) of the amine-reactive cross-linker DSG. Arrowheads indicate presumed α-Syn monomer (α-S1) and oligomers (such as α-S2 for dimeric α-Syn). B, lane 1, molecular weight marker (MW, SeeBlue plus2 prestained Standard, Invitrogen). Lanes 2–4, cross-linked α-Syn DOPS lipoprotein particles (final concentration 83 μm) with increasing concentrations of DSG as indicated. Presumed α-Syn monomer and oligomers are indicated by arrowheads. C, lane 1, MW, Precision Plus ProteinTM Dual Color Standard, BIORAD). Lanes 2–7, decreasing concentrations of α-Syn DOPS lipoprotein particles were exposed to a constant concentration of DSG (final concentration 249 μm). Lane 8, (−) non cross-linked α-Syn DOPS lipoprotein particles for control. D, approximately 9 μg monomeric α-Syn was loaded per well. Lane 1, (−) non cross-linked monomeric α-Syn control. Lanes 2–4, cross-linked monomeric α-Syn (final concentration 83 μm) with increasing concentrations of DSG. Lane 5, MW (Precision Plus ProteinTM Dual Color Standard, Biorad).