FIGURE 3.

Biophysical characterization of compound binding to the DENV RdRp. A, individual sensorgrams for the DENV-3 polymerase binding to increasing concentrations of JF-31-MG46, measured by surface plasmon resonance. B, the steady-state binding level in A is plotted versus the compound concentration, and the data are fitted to a single-site binding equation to calculate the equilibrium dissociation constant. C, isothermal titration calorimetry plot for PC-79-SH52 binding to the DENV-4 RdRp. The area under each of the peaks (an individual injection) was fitted to a single-site binding equation to determine the equilibrium association constant and binding enthalpy.