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. 2016 Feb 19;291(16):8549–8564. doi: 10.1074/jbc.M115.674200

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — The localization of GMFG in human monocytes. Human monocytes were seeded on FN-coated chambered coverglass for 20 min. Attached cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and stained with the indicated antibodies. A, confocal images show endogenous GMFG (green), Rab4 or Rab11 (red) (rapid and slow recycling markers), or β1-integrin (red) and nuclear DNA (blue; DAPI) in human monocytes. B, confocal images show GMFG distribution in human monocytes. Scale bar, 10 μm.