TABLE 2.
List of primers used for cloning and site-directed mutagenesis in this study
| Primer | Sequence (5′–3′) | Length |
|---|---|---|
| bp | ||
| TodST-F | AAA GGA TCC AGG AGA CAT ATG AGC TCC TTG GAT A | 34 |
| TodST-R | TAT AAG CTT CTA TTC CAG GCT ATC CTT | 27 |
| TodSTpp-u | TAT ATG CAT CTC GAG AAA CGA GCC CAG TAC | 30 |
| TodSTpp-m | CCC TCT AGA AGC TTG CTA TTA CCT CTC TTC CAC C | 34 |
| mexCn-u | AAA GAA TTC GCA AGA CAG GTT CGA TAA GGG TG | 32 |
| mexCn-d | AAA GGA TCC AAT GCG CCC GGA GAT CGG | 27 |
| mexCc-u | AAA GGA TCC GAT ATC CAG CAA CTC GAC CCG | 30 |
| mexCc-d | TTT GCA TGC GTT TCT GCG CAG GCG CAA CG | 29 |
| Sm-N | AAA GGA TCC AAG CTT GAA CCT TGA CCG AAC GCA | 33 |
| Sm-C | ATA GGA TCC TTA TTT GCC GAC TAC CTT GGT GAT C | 34 |
| PAS1(23) NcoI-F | CA ACC ATG GGA AAG GAG AAA GGA TCT GAA G | 30 |
| PAS1(43) NcoI-F | CA ACC ATG GCG CTC TAC GAG TTT GTG | 26 |
| PAS1(168) XhoI-R | CAA CTC GAG TCA CTC CAA TTC CTG GTT CTT C | 31 |
| TodS(23) BamHI-F | C AAG GAT CCG ATG AAG GAG AAA GGA TCT G | 29 |
| TodS(978) XhoI-R | CCG CTC GAG TGT GCC GGA GCC CTG TCT GG | 29 |
| L71M-F | TG GAG GGG GGC GGG ATT ACT ATG GAA GAA ATA CGA GGG AAG | 41 |
| L71M-R | CTT CCC TCG TAT TTC TTC CAT AGT AAT CCC GCC CCC CTC CA | 41 |
| F46A-F2 | GAT GGG CTC TAC GAG GCC GTG GGC CTT CTT GAT G | 34 |
| F46A-R2 | C ATC AAG AAG GCC CAC GGC CTC GTA GAG CCC ATC | 34 |
| V59A-F | CT CAT GGA AAT GTG CTT GAA GCG AAC CAG GTC GCA TTG GAG GG | 43 |
| V59A-R | CC CTC CAA TGC GAC CTG GTT CGC TTC AAG CAC ATT TCC ATG AG | 43 |
| A63V-F | G CTT GAA GTG AAC CAG GTC GTA TTG GAG GGG GGC GGG ATT A | 41 |
| A63V-R | T AAT CCC GCC CCC CTC CAA TAC GAC CTG GTT CAC TTC AAG C | 41 |
| F79Y-F | GAA ATA CGA GGG AAG CCA TAC TGG AAG GCG CGT TGG TGG | 39 |
| F79Y-R | CCA CCA ACG CGC CTT CCA GTA TGG CTT CCC TCG TAT TTC | 39 |
| W84V-F | G AAG CCA TTC TGG AAG GCG CGT GTG TGG CAA ATT TCA AAA AAA ACC | 46 |
| W84V-R | GGT TTT TTT TGA AAT TTG CCA CAC ACG CGC CTT CCA GAA TGG CTT C | 46 |
| W85H-F | CCA TTC TGG AAG GCG CGT TGG CAT CAA ATT TCA AAA AAA ACC GAG | 45 |
| W85H-R | CTC GGT TTT TTT TGA AAT TTG ATG CCA ACG CGC CTT CCA GAA TGG | 45 |
| W85R-F | CCA TTC TGG AAG GCG CGT TGG CGT CAA ATT TCA AAA AAA ACC GAG | 45 |
| W85R-R | CTC GGT TTT TTT TGA AAT TTG ACG CCA ACG CGC CTT CCA GAA TGG | 45 |
| I114V-F | GAA TTT GTT CGC TGT GAT GTT GAG GTT CTT GGA AAA TCA GGT GGA AGA G | 49 |
| I114V-R | C TCT TCC ACC TGA TTT TCC AAG AAC CTC AAC ATC ACA GCG AAC AAA TTC | 49 |
| V126A-F | CA GGT GGA AGA GAG GTA ATA GCC GCC GAT TTT TCA TTG CTG CCA ATT TG | 49 |
| V126A-R | CA AAT TGG CAG CAA TGA AAA ATC GGC GGC TAT TAC CTC TCT TCC ACC TG | 49 |
| F128L-F | GA GAG GTA ATA GCC GTC GAT CTT TCA TTG CTG CCA ATT TGC | 41 |
| F128L-R | GCA AAT TGG CAG CAA TGA AAG ATC GAC GGC TAT TAC CTC TC | 41 |
| A145V-F | G AGC ATT GTT TAC CTT CTT GTG GAA GGG CGC AAT ATT ACC G | 41 |
| A145V-R | C GGT AAT ATT GCG CCC TTC CAC AAG AAG GTA AAC AAT GCT C | 41 |
| L131D-F | ATA GCC GTC GAT TTT TCA TTG GAT CCA ATT TGC AAT GAA GAA GGG | 45 |
| L131D-R | CCC TTC TTC ATT GCA AAT TGG ATC CAA TGA AAA ATC GAC GGC TAT | 45 |
| V47L-F | GAT GGG CTC TAC GAG TTT CTG GGC CTT CTT GAT GCT C | 40 |
| V47L-R | G AGC ATC AAG AAG GCC CAG AAA CTC GTA GAG CCC ATC | 40 |
| L49D-F | GG CTC TAC GAG TTT GTG GGC GAT CTT GAT GCT CAT GGA AAT G | 42 |
| L49D-R | C ATT TCC ATG AGC ATC AAG ATC GCC CAC AAA CTC GTA GAG CC | 42 |
| E58A-F | GCT CAT GGA AAT GTG CTT GCA GTG AAC CAG GTC GCA TTG | 42 |
| E58A-R | CAA TGC GAC CTG GTT CAC TGC AAG CAC ATT TCC ATG AGC | 42 |
| E146A-F | C ATT GTT TAC CTT CTT GCG GCA GGG CGC AAT ATT ACC GAT AA | 42 |
| E146A-R | TT ATC GGT AAT ATT GCG CCC TGC CGC AAG AAG GTA AAC AAT G | 42 |
| E146L-F | C ATT GTT TAC CTT CTT GCG CTG GGG CGC AAT ATT ACC GAT AA | 42 |
| E146L-R | TT ATC GGT AAT ATT GCG CCC CAG CGC AAG AAG GTA AAC AATG | 42 |
| R148A-F | C CTT CTT GCG GAA GGG GCA AAT ATT ACC GAT AAG AAG | 37 |
| R148A-R | CTT CTT ATC GGT AAT ATT TGC CCC TTC CGC AAG AAG G | 37 |
| R148M-F | C CTT CTT GCG GAA GGG ATG AAT ATT ACC GAT AAG AAG | 37 |
| R148M-R | CTT CTT ATC GGT AAT ATT CAT CCC TTC CGC AAG AAG G | 37 |
| Y619A/E620A-F | GCG CGT TGG AAA GCA GTG GCC GCC AAC TCT GCG GCC GGT ATT G | 44 |
| Y619A/E620A-R | C AAT ACC GGC CGC AGA GTT GGC GGC CAC TGC TTT CCA ACG CGC | 43 |
| Δ617–623-F | CCG CCT CGG AAG CGC GTT GGA AAG CCG GTA TTG TAC TGA CCG ACC | 45 |
| Δ617–623-R | GGT CGG TCA GTA CAA TAC CGG CTT TCC AAC GCG CTT CCG AGG CGG | 45 |
| E666A-F | CTG ACT CCA TCT GAC GCA AGC CCA CAG ATA AAG C | 34 |
| E666A-R | G CTT TAT CTG TGG GCT TGC GTC AGA TGG AGT CAG | 34 |
| L674A-F | CAG ATA AAG CAG CGT GCA GCC AAT TTG CTT CAG | 32 |
| L674A-R | CTG AAG CAA ATT GGC TGC ACG CTG CTT TAT CTG | 32 |
| Y691I-F | C AGT GTG GAG CGC TCC ATT CTA TGC AAA AAT GGT TC | 36 |
| Y691I-R | GA ACC ATT TTT GCA TAG AAT GGA GCG CTC CAC ACT G | 36 |
| A703S-F | CT ACG ATT TGG GCC AAT TCG AGT GTC TCG CTG ATG | 35 |
| A703S-R | CAT CAG CGA GAC ACT CGA ATT GGC CCA AAT CGT AG | 35 |