FIGURE 7.

AUC analysis of the TSC1-TBC1D7 interaction. TSC1, TBC1D7, and mixtures of TBC1D7 and TSC1 with two different molar ratios were measured by analytical ultracentrifugation analysis using the sedimentation velocity method. TSC1 and TBC1D7 alone formed monomers. When TSC1 was in excess, the TSC1-TBC1D7 complex formed predominantly a 1:2 heterotrimer. In contrast, when TBC1D7 was in excess, 2:2 heterotetramers were also detected. All AUC measurements were performed at least twice, and all results were highly reproducible.