FIGURE 5.

Complementation of E. coli growth in the presence of 100 μm mupirocin by expression of ScIleRS or SgIleRS in various conditions. In all experiments, the expression of ScIleRS, SgIleRS, or EcIleRS from pET28 plasmid was induced by 50 μm isopropyl 1-thio-β-d-galactopyranoside. A, complementation of E. coli growth on LB plates by expression of additional EcIleRS, ScIleRS, or SgIleRS. Semi-quantitative complementation drop-test of E. coli growth was performed by adding 100-μl decimal dilutions (−1 to −6) of each strain to the following plates. B, LB plates with or without mupirocin and M9 plates (no amino acids added) with or without mupirocin. C, M9 plates supplemented with 150 μm Ile, 150 μm Leu, and 1, 4, or 10 mm Val with or without mupirocin.