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Activation of isoleucine and valine by SgIleRS and ScIleRS at 30 °C
The values represent the best fit value ± S.E. of at least two independent experiments.
| Km/μmol dm−3 | kcat/s−1 | kcat/Km/s−1 μmol dm−3 | Discrimination factor | |
|---|---|---|---|---|
| SgIleRS + Ile | 17.4 ± 3.0 | 20.6 ± 1.4 | 1.18 | |
| SgIleRS + Val | 1750 ± 130 | 19.3 ± 0.4 | 0.01 | 118 |
| ScIleRS + Ile | 7.6 ± 0.3 | 11.4 ± 0.8 | 1.5 | |
| ScIleRS + Val | 840 ± 10 | 8.6 ± 1 | 0.01 | 147 |
