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. 2016 Jan 14;291(16):8644–8652. doi: 10.1074/jbc.M115.694208

FIGURE 4.

FIGURE 4.

Overexpression of GCNF induced the differentiation of hES cells. A, rescue experiment diagram: G-hES cells were treated with Dox, RA, and Dox plus RA for 4 days, and then cultured without treatment factors for another 4 days. B, Western blot results of GCNF and Oct4 expression in G-hES cell cultures treated with or without Dox, RA, and Dox plus RA for 4 days, and expression after those treatments were removed for another 4 days. C, analysis of qRT-PCR results of GCNF expression in rescue experiments. D, analysis of qRT-PCR results of Oct4 expression in rescue experiment. E, Western blot results of GCNF and Oct4 expression in G-hES cell cultures at day 4 or day 8 in UM or DM that included RA or RA plus Dox. F, morphological changes after G-hES cells were treated with Dox, RA, and Dox plus RA for 8 days. Untreated G-hES cells were used as control. Scale bar: 50 μm. UM stands for medium used for growth of undifferentiated cells; DM stands for medium used for growth of differentiated cells. * indicates no statistical significance with p ≥ 0.05; ** indicates statistical significance with p < 0.05.