FIGURE 8.

Inhibition of NOXO1 restores eNOS activity uncoupled by OSS. siRNA against NOXO1 was used to examine whether OSS-induced changes in superoxide and NO production are related to the uncoupling of eNOS. Bovine aortic endothelial cells were treated with control or NOXO1 siRNA prior to being subjected to static or OSS conditions. Superoxide was measured from these cells in the presence or absence of L-NAME, a NOS inhibitor, to assess the coupling state of eNOS. Obviously, L-NAME inhibited superoxide production under OSS conditions, implicating uncoupling of eNOS. This response was fully reversed by the treatment with NOXO1 siRNA (n = 3); *, p ≤ 0.05.