FIGURE 9.

The rates of maturation of R381Q and G149R are reduced compared with that of the common variant IL23Rα. A, HEK293 cells stably expressing IL23Rα and IL23Rα variants were treated with cycloheximide (CLX, 500 μm) for 4 h followed by washing off the cycloheximide and incubated for the indicated time points. Representative Western blot analysis of the cell lysates is presented. B, synthesis of IL23Rα mature (M) and immature (IM) levels over time after cycloheximide exposure are represented in pixels. Linear regression analysis of the data were used to calculate the rate using GraphPad Prism 6.0, and the R² value for the receptors are as follows: IL23Rα M (0.8591), IL23Rα IM (0.7436), R381Q M (0.9256), R381Q IM (0.9054), G149R M (0.8357), G149R IM (0.9543), V362I M (0.8428), V362I IM (0.8117). The error values are S.E. where n = 3. C, ratio of the rate for IL23Rα and variants between their mature and immature receptor synthesis is represented here. Error values are S.D. where n = 3. Statistical significance calculated by ANOVA is denoted by the asterisk (*) compared with IL23Rα, where p < 0.01. In addition, R381Q was significantly different from V362I, with a p < 0.01, and G149R was significantly different from V362I, with a p < 0.01.