TABLE 3.

pA₂ of antagonists for cAMP production mediated by CTR and AMY₁ receptors

NS, no significance compared with the template peptide used for mutagenesis. Analysis of variance with Tukey's post hoc test was used for statistical analysis.

Peptide	CTR with hCT agonist			AMY1 with rAmy agonist			AMY1 with hCT agonist
	n	Mean	S.E.	n	Mean	S.E.	n	Mean	S.E.
AC413(6–25)	3	7.04	0.03	7	7.43	0.09	4	7.19	0.11
AC413(6–25) Y25P	3	8.48a	0.01	4	8.03a	0.08	4	8.71a	0.11
AC413(6–25) Y25A	3	7.16NS	0.16	4	6.53a	0.08	3	7.31NS	0.15
rAmy(8–37)	3	<5.2		3	5.98	0.09	3	<5.2
rAmy(8–37) Y37P	3	6.73	0.02	3	7.52b	0.11	3	6.83	0.10
rAmy(8–37) Y37A	3	5.33	0.11	3	6.02NS	0.03	3	5.46	0.14

^a p < 0.05 compared with AC413(6–25).

^b p < 0.05 compared with rAmy(8–37).