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Effect of FCCP and nigericin on [pH]c. FCCP induces a dose-dependent reduction in pH of SHSY5Y neuroblastoma cells (A and B). 10 μm FCCP induce a profound decrease of intracellular pH compared with 1 μm of this protonophore. C, changes in [pH]c of SH-SY5Y neuroblastoma cells in response to subsequent application of the different concentrations of nigericin and FCCP. D, dose-dependent curves of the effects of nigericin and FCCP on intracellular pH of SH-SY5Y neuroblastoma cells.
