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. 2016 Feb 18;291(16):8701–8708. doi: 10.1074/jbc.M115.691774

FIGURE 3.

FIGURE 3.

Sources of FCCP- and nigericin-induced acidification of cytosolic pH of SH-SY5Y cells. Reduction of extracellular pH (HBSS) from 7.4 to 6.35 did not change the effect of 10 μm FCCP (A) but reduces the effect of nigericin (B). Depolarization of mitochondrial membrane with 0.2 μg/ml oligomycine + 5 μm rotenone (C) but not oligomycine alone (D) had an effect on pH changes induced by 10 μm FCCP. 5 μm antimycin A (E) or 1 μm FCCP (F) decrease intracellular pH and reduce the effect of 3 μm nigericine. G, effect of 20 μm iodoacetic acid on action of 10 μm FCCP on [pH]c. H, inhibitor of Na+/H+ exchanger DMA had no effect on cytosolic pH in concentrations of 10 and 50 μm.