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. 2016 Feb 23;291(16):8756–8772. doi: 10.1074/jbc.M115.704973

FIGURE 1.

FIGURE 1.

ZSCAN21 expression in different brain areas from embryonic and adult rats. Different brain areas were isolated from embryonic (E17) and adult rats. The brain areas isolated were: midbrain (MID) or midbrain dorsal (MD) and midbrain ventral (MV), striatum (STR), olfactory bulbs (OB), frontal cortex (FC), cerebellum (CER), and hippocampus (HIP). Following homogenization, RNA or protein isolation was performed. Α, RT-PCR for ZSCAN21 and SNCA. β-Actin, loading control; −, negative ctl/no sample; +, PC12 cell RNA extract. B, specificity of the Genscript ZSCAN21 polyclonal antibody. The specificity of the ZSCAN21 antibody was verified following competition with the ZSCAN21 blocking peptide (SNLTKHRRTHTGEKPY, synthesized by Genscript). As a positive control region for ZSCAN21 expression, we used a cerebellum lysate and as negative control a liver lysate. Western blot for ZSCAN21 and SNCA. ERK, loading ctl. Expression of ZSCAN21 (mRNA, protein) was detected in all tested brain areas, both embryonic and adult. Similarly, the expression of SNCA was also verified as expected.