FIGURE 2.

CBS peptide inhibits the binding of mCRP to various types of ligands. ApoB (A), C1q (B), fibronectin (C), collagen IV (D), fibrinogen (E) in TBS or cholesterol (F) in ethanol was immobilized onto microtiter wells (n = 3–4). mCRP at different concentrations was added and binding was evaluated using mCRP-specific mAb 3H12 (left panel). The half-saturated binding concentration of mCRP was selected to test the inhibitory effects of co-added peptides (middle panel). The peptide of CBS (a.a. 35–47) emerged as the most potent inhibitor against all interactions. The IC₅₀ of CBS or CBS in multiple antigenic peptide (MAP) format with 4 branches was further determined (right panel).