FIGURE 1.
The unique lysine residue of NY-ESO-1 at position 124 is not essential for NY-ESO-1157–165 presentation. A, HeLa cells were subjected to 24-h transfection with HLA-A*0201 together with either NY-ESO-1 or NY-ESO-1K0. The NY-ESO-1157–165 CTL response was assessed using the CTL clone RG39 specific for NY-ESO-1157–165 at various effector:target ratios as indicated. Internal controls in this experiment consisted of HLA-A*0201-expressing HeLa cells or cells loaded with 10 μm of the 9-mer SLLMWITQV synthetic peptide. After 16 h of co-culture, supernatants were tested for their IFN-γ content by ELISA. The results are expressed as mean ± S.D. of duplicate values. Shown is one representative experiment of three. B, NY-ESO-1 expression was analyzed by RT-PCR (top panel) and Western blotting (bottom panel) as indicated. IB, immunoblot.