FIGURE 5.

FoxO1 knockdown does not impact C/EBPα/β binding to the IGFBP1 and G6Pase promoters. A–D, ChIP analysis was performed on chromatin isolated from unstimulated/insulin-stimulated HepG2 cells infected with scrambled or FoxO1 shRNA with antibodies directed against C/EBPα (A and C) or C/EBPβ (B and D). Results are normalized to total input, and values are expressed as a ratio over non-insulin-treated cells infected with the scrambled shRNA. Data represent the mean ± S.E. (error bars) for three independent biological experiments and two technical replicates per biological replicate.