FIGURE 2.

Conditional deletion of Cdc42 in mammary glands inhibits normal alveologenesis during lactation. Whole mount images of mammary glands using Carmine Red staining to visualize the epithelial network in control and CCKO mice in the virgin stage (a, b), and at lactation day 20 (c, d). e, f: insets from c, d with arrowheads to indicate fully expanded alveoli in control mice, or latent alveoli in CCKO mice. g, h: Fast Green and Sirius Red staining depicting the epithelial and collagen basement membrane structures in alveoli sections, respectively. Arrowhead in h indicates abnormally sloughing off of an epithelial cell. Scale bars represent 50 μm. The results are representative of the analysis of >3 mice each from control and CCKO groups.