FIGURE 5.

In vitro aggregation of synthetic TDP-43 peptides (40 amino acid residues). A, schematic diagrams of deletion sites on TDP-43 and sequences of synthetic peptides. B, TDP-43 peptide solutions of 0.05, 0.1, and 0.2 mm were prepared and incubated in 37 °C on a shaker. At the indicated time point, aliquots of peptide solution were added to 5 μm ThS solution, and ThS fluorescence was measured. Data are means ± S.E. (n = 3). C, peptide solutions after 8-day incubation at 37 °C on a shaker were loaded on a grid and negatively stained with phosphotungstate. Samples were observed under a transmission electron microscope. Magnification of acquired micrographs is ×40,000, and scale bar is 100.0 nm.