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. 2015 Dec 30;291(17):8931–8950. doi: 10.1074/jbc.M115.695304

FIGURE 4.

FIGURE 4.

The effects of trans-presented IL-15:IL-15Rα and encapsulated antigen require coordinated delivery to DCs and T cells. To investigate the requirements for the adjuvant effect of IL-15:IL-15Rα, DCs were compared in their ability to stimulate OT-I CD8+ T cells under different conditions of IL-15:IL-15Rα and OVA delivery. a, the requirement for both particle types to go to the same cell was tested by mixing DCs that were separately given IL-15/NP or NP/OVA and comparing them with DCs given IL-15/NP/OVA. b, the effect of priming CD8+ T cells with IL-15 constructs was tested by priming CD8+ T cells overnight with free or NP-bound IL-15:IL-15Rα and stimulated with NP/OVA-DCs. c, to test the requirement for OVA or IL-15 to be delivered on the same particle, DCs were incubated with different particle constructs overnight and then used to activate OT-I CD8+ T cells. In all cases, the T cell response was compared against untreated DCs and IL-15/NP/OVA-treated DCs. OT-1 cell counts were determined as a measure of total stimulatory capacity of each group. Data were analyzed by a one-way ANOVA and a Tukey post-hoc test. * indicates a significance level of p ≤ 0.05, ** p ≤ 0.01, and *** indicates p ≤ 0.001.