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Immunoblot analyses for PKM1, PKM2, and PKLR in the lysates of SGC7901, Bcap37, HeLa, 4T1, RKO, SMMC7721, and HepG2 cells and recombinant PKM1, PKM2, and PKL were used as positive control. The amount of cell lysate protein was 10 μg and recombinant protein was 2 μg. The experiments were repeated two times.
