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Activities of HK, PFK1, and PKM2 assayed according to the substrate concentration in cancer cells. A, actual activities of enzymes. The measurement of enzyme activity is described under “Experimental Procedures” under the subtitle “Measurement of Enzyme Activities at Physiological Substrate Concentration.” B, activities of PKM2 or PFK1 relative to HK, which is defined as 1. Data are means ± S.D., n = 3.
