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. 2016 Mar 1;291(17):9000–9013. doi: 10.1074/jbc.M116.718973

FIGURE 4.

FIGURE 4.

Modification of the H3K4me3 peptide affects binding. A, ITC curve of CW domain of ZCWPW2 binding after N-terminal alanine addition of H3K4me3 peptide. B, ITC curve of CW domain of ZCWPW2 binding after N-terminal alanine deletion from H3K4me3 peptide. C, ITC curve of CW domain of ZCWPW2 binding to histone H3 R2A H3K4me3 peptide. D–F, ITC curves of CW domain of ZCWPW2 binding after H3R2 methylation of H3K4me3 peptide, monomethylation (D), symmetric methylation (E), and asymmetric methylation (F).