FIGURE 5.
SUMO1 overexpression affects TDG sumoylation but not TDG-mediated repair of 5caC. A, cells were transfected with expression vectors for wild type TDG, Myc-SUMO1, or both vectors, as indicated. Protein expression and TDG sumoylation was monitored by immunoblot analysis. B, cells were transfected with expression vectors for TETCD together with vectors for wild type TDG, SUMO1, or both vectors, as indicated. Protein expression and 5caC levels were monitored by immunoblot analysis. ** indicates sumoylated TDG. Protein and DNA loading were assessed by tubulin and methylene blue staining, respectively.