TABLE 5.

Thermodynamic parameters of the interactions between the UBA5 LIR/UFIM peptide and human UBLs

	ΔH	ΔS	−T*ΔS	ΔG	Ka × 106	Kd	n
	kcal mol−1	cal mol−1 K−1	kcal mol−1	kcal mol−1	m−1	μm
LC3A	−1.08 ± 0.04	+18.0	−5.36	−6.44	0.054 ± 0.005	18.5	1a
LC3B	+3.84 ± 0.51	+30.0	−8.94	−5.10	0.006 ± 0.001	179	1a
LC3C	−1.61 ± 0.02	+16.0	−4.77	−6.38	0.048 ± 0.002	20.7	1a
GABARAP	−2.51 ± 0.06	+13.8	−4.11	−6.62	0.070 ± 0.003	14.4	1.03 ± 0.02
GABARAPL1	−3.53 ± 0.16	+11.9	−3.55	−7.08	0.157 ± 0.015	6.4	0.96 ± 0.03
GABARAPL2	−5.91 ± 0.09	+6.6	−1.97	−7.88	0.598 ± 0.045	1.7	0.98 ± 0.01
UFM1	−1.51 ± 0.04	+18.2	−5.42	−6.93	0.123 ± 0.069	8.1	1.10 ± 0.02

^a The n value was fixed to 1.00 upon fitting.