FIGURE 3.

Osmotic swelling potentiates RA-MA currents in HEK293 cells heterologously expressing Piezo2 channels. A, sample traces of Piezo2-mediated RA-MA currents (Piezo2 currents) recorded from a Piezo2-expressing HEK293 cell at initial time and 15 min after establishing the whole-cell mode with hypertonic recording internal solution. A membrane displacement step of 8 μm is indicated above the current traces. B, summary data of Piezo2 currents over time after establishing the whole-cell mode with hypertonic (circles, n = 5) or isotonic (squares, n = 6) recording internal solution. C, two sets of sample traces of Piezo2 currents elicited by different membrane displacement steps. The Piezo2 currents were recorded at the initial time (top) and 20 min (bottom) after establishing the whole-cell mode with hypertonic recording internal solution. Membrane displacement steps in the increment of 1 μm are indicated above the traces. D, summary data of the Piezo2 currents evoked by different membrane displacement steps. Piezo2 currents were recorded at initial time (squares, n = 8) and 20 min (circles, n = 8) after establishing the whole-cell mode with hypertonic recording internal solution. E, decay time constants of Piezo2 currents over time after establishing the whole-cell mode with hypertonic (closed bars, n = 7) or isotonic (open bars, n = 5) recording internal solution. F, mechanical thresholds for eliciting Piezo2 currents at initial time and 20 min after establishing the whole-cell mode; the recordings were made with hypertonic (closed bars, n = 8) or isotonic (open bars, n = 6) recording internal solution. Data represent mean ± S.E., *, p < 0.05; **, p < 0.01; ***, p < 0.001.