FIGURE 5.

Gβ₅ is required for stability but not for homo-oligomerization of RGS7. A, HEK293T cells expressing FLAG-, YFP-, or mCherry-tagged RGS7 with or without Gβ₅ were lysed and analyzed by Western blot using antibodies against RGS7, Gβ₅, and actin. The white arrow shows FLAG-RGS7 expressed in the absence of Gβ₅. B, cells on coverslips were transfected with YFP-RGS7 with or without CFP-Gβ₅, then fixed, and YFP and CFP direct fluorescence was detected using a confocal microscope (×63 objective lens). C, lysates from cells expressing YFP-RGS7 and mCherry-RGS7 with or without Gβ₅ were subjected to immunoprecipitation using immobilized YFP antibody. The eluates were analyzed by Western blot using YFP, Gβ₅, and mCherry antibodies. The black arrow points to the co-immunoprecipitated mCherry-RGS7.