TABLE 1.

Steady-state kinetic parameters and Bond lengths and molecular dipoles of substrates

Steady-state kinetic parameters show relatively high substrate affinity for PcrAB in comparison with EccNarGHI. A, wild-type PcrAB; B, EccNarGHI; C, PcrA W461E. The steady-state kinetics were determined using the NADH/PMS assay. Error represent one standard deviation of triplicate assays. D, Bond lengths and molecular dipoles of substrates (52). N.S., not a substrate. *, Iodate was also previously determined to not be a substrate of EccNarGHI (17). †, Estimated values due to the solubility limitations of NaIO₃.